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ABSTRACT
A hybrid cloning procedure for construction of gene silencing transformation vectors, involving intron-hairpin structures, was developed. The intron-hairpin structure was initially assembled through PCR-amplification and digestion/ligation of the target gene and intron regions. The obtained ihp-fragments were directly cloned into plant transformation vectors or Gateway™ entry vectors employing a BP Clonase® reaction of the Gateway™ cloning system.