The Effects of Biotic and Abiotic Elicitors on the Capsidiol Accumulation in Cell Suspension Cultures of Capsicum Annuum L.

ABSTRACT

The effects of Alternaria alternata (Fr.) Keissler, yeast extract, cellulase, jasmonic acid (JA), hydrogen peroxide (H₂O₂), and salicylic acid (SA) on the capsidiol accumulation were investigated in the cell suspension cultures of three genotypes (resistant, PM 217 and PM 702; susceptible 11 B 14) of Capsicum annuum L. which have different resistant to Phytophthora capsici Leon. Cell suspension cultures were obtained from hypocotyle expiants of pepper seedlings emerged under in vitro conditions. The two-week old green pepper suspension cultures were elicited using auto-claved hyphae suspension of A. alternata, yeast extract, cellulase, JA, H₂O₂ and SA elicitors. The capsidiol concentration was determined by gas chromatograhy analysis, 24 and 48 h after elicitation. The amounts of capsidiol accumulated in the cultures with elicited cellulase and A. alternata were higher than the other elicitors. Besides a negligible amount of capsidiol accumulation was observed with H₂O₂ and nothing with SA in the concentration range studied. Maximum capsidiol concentration was obtained as 91.42± 3.9 mg/mL in the cultures of 11 B 14 susceptible genotype elicited with 1500 mg/mL cellulase at 24 h later. The capsidiol concentrations were high for resistant genotypes (PM 217 and PM 702) in the cultures with elicited A. alternata and yeast extract. But the capsidiol concentrations were high for susceptible genotype(11 B 14) in the cultures with elicited cellulase, JA, H₂O₂ and SA.