Plant Regeneration via Direct Organogenesis and Somatic Embryogenesis of two new Bulgarian Spray Carnation Cultivars

ABSTRACT

Conditions for plant regeneration of leaf expiant from Bulgarian spray carnation cultivars (Fea and Rossitza) were established. Solid MS medium supplemented with BAP and NAA was used for direct induction of adventitious shoots. The optimal medium for regeneration for both cultivars contained 0.9 mg/l BAP and 0.9 mg/l NAA. The mean number of regenerants per explant varied 7–8 for tested cvs. Regenerated plants possessed normal phenotype. At dark and light conditions induction of embryogenic callus were observed for both cultivars. Vigor somatic embryos were obtained on embryo formation medium supplemented with 0.05 mg/l BAP and 250 mg/l casein hydrolysate. They developed to plants with normal phenotype and rooted easily at in vitro condition.