ABSTRACT
Broomrapes (Orobanche spp.) are the most devasting parasitic weeds, causing extensive damage to many important agricultural crops worldwide. Despite the tremendous economic impact of broomrapes on the agriculture little is known of the pattern of the genetic variation within this group until now and particularly in Bulgaria and other South-Eastern European countries. The present paper describes the application of six RAPD markers for the study of Orobanche species—O. cumana Wallr./O. cernua Loefl. and O. ramosa L. using seed samples originating from different agro-ecological regions in South-Eastern Europe and middle East. Genetic variation among some of O. cumana Wallr./O. cernua Loefl. samples representing populations originating from different geographical regions was detected.