New Technique: Measurement of Microleakage Volume in the Marginal Gaps of the Dental Restorations

ABSTRACT

This study investigates the feasibility of a different approach to determining the microleakage volume associated with dental restorations and the relative marginal adaptation deficiency of dog, bovine and human primary teeth in in vitro conditions. It was also researched the appropriateness of using dog and bovine teeth in in vitro studies instead of human teeth. Our method utilizes the molecular characteristics of methylene blue (MB). Within the framework of this study, 60 permanent teeth (20 human, 20 dog and 20 bovine) were used. Class V cavities were prepared in all human, dog and bovine primary teeth and all restorations were made by Composite. These groups were evaluated statistically for dye penetration and microleakage volume measurements using Kruskal-Wallis non-parametric test (p>0.05) and One Way ANOVA, both of which indicated no statistically significant differences (p>0.05). This preliminary investigation showed that the new microleakage volume measurement method might be a valuable tool for the in vitro study of microleakage dynamics around dental restorations. However, this method can be used as a new technique for the determination of microleakage volume. As a result of this study; dog and bovine teeth can be used instead of human teeth.