On-Line State and Parameters Estimation of the Model for Glucoamylase Production using Aspergillus Niger Cells

ABSTRACT

Starch-degrading enzymes of microbial origin have a number of industrial applications. The mould extracellular enzyme, glucoamylase, is of major importance in the starch industry in the commercial production of considerable significance in an industrial context since it offers the advantages of increased reaction rates, decreased viscosity, reduced microbial contamination and better storage stability. Fungal glucoamylase are of industrial importance in production of sugar from starch. Most glucoamylases are produced today by submerged fermentation with Aspergillus niger strains. A general chemostat microbial cultivation model is used. The estimation procedure is based on the extended Kalman filtering method. Thus the necessity of performing experiments for the sake of parameter identification could be successfully avoided. In this case-study a batch process of glucoamylase preparation is studied from a mutant strain Aspergilus niger B-77. The estimation performance is studied under different initial conditions.