Microsatellite Studies of the Sphaerococcum Mutant Forms

ABSTRACT

Genomic polymorphism of sphaerococcum mutant forms№613, 6512, 6x-Triticale MT47 and their derivative forms 49Lsph, 49Laest., 49L/13sph., 2k x 613sph, were investigated by means of microsatellite markers of 3D, 3B and 1B chromosomes. The present investigation was undertaken to resolve the intricacies related to the complex character of sphaerococcum mutant forms. Within the range of the screened mutant and their control forms more than two alleles were detected at the five microsatellite loci. For each microsatellite locus, a screen over mutant forms was performed to find the typical alleles corresponding to the mutant and control forms. Five of the microsatellite loci 161(3D), 52(3D), 533(3B), 493(3B) 131–1b(3B) and 131(1B) showed clear cut allelic differences (one or two allele) respectivelly genomic polymorphism between sphaerococcum mutant foms their derivatives as well as their control forms. On the base of the microsatellite data a dendrograme of the mutant and control forms was constructed. Our results demonstrate that microsatellites can be successfully used for studing genetical polymorphism and identification of different sphaerococcum mutant forms.