Visualization of Secale Cereale DNA in Wheat Germ Plasm by Genomic In Situ Hybridization

ABSTRACT

Total genomic DNA from rye was labelled with biotin and used as a probe for in situ hybridization to show the sizes and translocation points of the rye chromosome segments in wheat mutant forms K1 and K2, which carry a translocation between wheat chromosome 1B, and the short arm of rye chromosome 1R (1B/1R). The translocation breakpoints were at or very near to, the centromere. Total genomic rye DNA used as a probe was also able to distinguish 12 rye chromosomes in 6x triticale-MT47 by genomic in situ hybridization. The techniques using genomic probes are useful for detecting, characterizing and following alien chromosomes or chromosome segments by genomic hybridization- GISH.