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ABSTRACT
Both indicator and serological methods were used to identify tobacco rattle virus (TRV) in sugar beet in Bulgaria. In mass screening of sugar beet crops, of 440 samples analyzed, 272 (62%) were positive. Beet TRV isolates were identified by the symptoms of 17 types of indicators and hosts of different botanical families. Of the experimental TRV hosts, wheat, barley and potato are the most important for our country. Serologically, the virus was identified by means of indirect (ACP) and DAS-ELISA with different TRV antisera. Tobacco rattle virus, isolated from sugar beet, is immunologically related to the typical virus strain (TRV type strain, potato isolate K), originating from potato—TRV K.