Duplex Polymerase Chain Reaction (PCR) for the Simultaneous Detection of Cryia(B) and the Maize Ubiquitin Promoter in the Transgenic Rice Line Kmd1

R. BabekovaBavarian Health and Food Safety Authority, Oberschleissheim, Germany;Technical University of Munich, Department of Food and Nutrition, Freising-Weihenstephan, Germany;National Center of Public Health Protection, Sofia, BulgariaCorrespondence[email protected]

T. FunkBavarian Health and Food Safety Authority, Oberschleissheim, Germany

S. PecoraroBavarian Health and Food Safety Authority, Oberschleissheim, Germany

K.-H. EngelTechnical University of Munich, Department of Food and Nutrition, Freising-Weihenstephan, Germany

D. BaikovaNational Center of Public Health Protection, Sofia, Bulgaria

U. BuschBavarian Health and Food Safety Authority, Oberschleissheim, Germany

ABSTRACT

This study describes a combination of screening and gene-specific methods for the detection of GMO in food and feed. Using specific primers, the maize ubiquitin promoter and the cryIA(b) gene, which encodes for a delta-endotoxin transferring insect resistance, can be detected simultaneously by a duplex PCR in samples containing the transgenic rice line Kemingdao1 (KMD1). This method is also suitable for the screening for Bt11 maize.

Keywords:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Duplex Polymerase Chain Reaction (PCR) for the Simultaneous Detection of Cryia(B) and the Maize Ubiquitin Promoter in the Transgenic Rice Line Kmd1

Information for

Open access

Opportunities

Help and information

Duplex Polymerase Chain Reaction (PCR) for the Simultaneous Detection of Cryia(B) and the Maize Ubiquitin Promoter in the Transgenic Rice Line Kmd1

ABSTRACT

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature