Effect of Auxin Type and Concentrations in Different Media on the Callus Induction and Shoot Formation of Crested Wheatgrass (Agropyron Cristatum (L.) Gaertn)

ABSTRACT

Effects of different medium (LS and SH) supplemented with various auxin concentrations (2, 4, 6 and 8 mg. l⁻¹) of dicamba (3,6-dichloro-2metoxibenzoic acid) and picloram (4-amino-3,5,6-trichloropicolinic acid) on the callus induction, direct shoot formation rate and callus weight from young inflorescences crested wheatgrass (Agropyron cristatum (L.) Gaertn).

The result of the study showed that callus induction rate (%), shoot formation (%) and callus weight (mg/per Petri dishes) from the young inflorescences were significantly affected by medium, auxin and auxin concentrations. Depending on the medium, mean callus induction rate, shoot formation and callus weight (mg/per Petri dishes) varied from 18% - 18.8%, 8.6% - 62.5%, 11.4 - 16.4 mg, respectively.

Callus induction, shoot formation rates and callus weight were also significantly influenced by the auxin type and auxin concentrations. The highest callus induction rate and callus weight were obtained from the LS medium containing 4 mg. l^−l of dicamba. The highest direct shoot formation rate was obtained from the SH medium containing 2 mg. l^−l of picloram.

Keywords:

• Crested wheatgrass
• young inflorescences
• in vitro culture
• auxin
• medium