Amendment of the Activity of Microbial Preparation Laktazym to Protein Biodegradation for Dairy Wastewater

ABSTRACT

There exist various biological products in the markets for augmentation of wastewater treatment process, but their specificity toward definite substrates is low. In the present study the amendment and the enrichment of biodegradation activity of commercial preparation Laktazym toward a target contaminant in dairy wastewater were investigated. Two microbial dominants—AS-05 and LB-16 with high biodegradation activity to the milk proteins were isolated by the applied selection pressure with casein. Both cultures were detailed characterized by biomass accumulation, effectiveness of organics, protein and lactose removal, as well as by key enzyme activities—total dehydrogenase, phosphatase and nitrate reductase.

Anaerobic biodegradation process for dairy wastewater in a critical situation of destabilized biofilm has been simulated in lab with different biological systems. The experimental data showed greater positive effect on the biodegradation of the organic matter, particularly of the milk proteins for the two modifications of investigated preparation with the selected cultures, than for the variant Laktazym. Moreover at the 48^th and the 72^nd hour the effectiveness of protein removal for the combination Laktazym + AS-05 (Peptostreptococcus sp.) was higher with 67%, than for the variant only with Laktazym.

Keywords:

• milk protein
• biodegradation activity
• dairy wastewater
• microbial preparation
• anaerobic treatment
• destabilized biofilm