692
Views
9
CrossRef citations to date
0
Altmetric
Original Articles

Validation of Lab-on-Chip Capillary Electrophoresis Systems for Total RNA Quality and Quantity Control

, &
Pages 829-834 | Published online: 15 Apr 2014
 

ABSTRACT

Purity and good RNA quality are important elements for the overall success of RNA based analysis methods like microarrays and real time qRT-PCR. There are two commercially available automated systems—the Experion (Bio-Rad Laboratories) and the 2100 Bioanalyzer (Agilent Technologies)—that provide both RNA sample quality and quantity analysis. In this study different aspects like the reproducibility and sensitivity of both systems were analyzed by determining the total RNA quality and quantity extracted from various bovine tissues. Regarding quantitation, the Experion is more sensitive than the 2100 Bioanalyzer. Both systems overstate the concentration by 19–29% compared to the photometric values. For RNA quality determination, both systems show highly comparable reproducibility. With the RNA integrity number (RIN) the 2100 Bioanalyzer offers an additional opportunity to quantify the RNA quality.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.