In Vitro Plant Regeneration from Hungarian Vetch (Vicia Pannonica Crantz) Using Cotyledonary Node Explants

ABSTRACT

Hungarian vetch is an important forage plant of Southern Europe and South Western Asia. It is progressively gaining popularity as forage plant among farmers of Turkey. In vitro plantlet regeneration in the plant was achieved from cotyledonary node explants on MS medium containing various concentrations of TDZ. The results showed that TDZ concentrations affected frequency of shoot regeneration, mean number of shoots per explants and shoot length in the range of 66.67–100%, 3.28–9.33 and 1.28–3.84 cm respectively. The maximum number of shoots was recorded on MS medium containing 0.45 mg/l TDZ. These were rooted by pulse treatment with 50 mg/l IBA for 7 min and then culturing on 1/2 concentration of MS micro and macro salts and vitamins or MS medium containing 0.30, 0.60, 0.90, 1.20 or 1.60 mg/l IBA. Rooting was more successful on non pulse treated shoots. All of them were put into pots, transferred to growth chamber and then to fields, where they flowered and set seeds.

Keywords:

• Hungarian Vetch
• Vicia pannonica
• cotyledonary node
• Thidiazuron
• indole-3-butyric acid
• axillary shoot regeneration
• rooting
• pulse treatment