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Short Communication

Rapid and Efficient Method for Production of T4 Endonuclease V by Heterologous Expression in E.Coli

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Pages 1011-1012 | Published online: 15 Apr 2014
 

ABSTRACT

T4 endonuclease V is specific to cyclobutane-pyrimidine dimers produced by UV-irradiation. When applied topically in mammals, it induces strand breaks in the altered DNA, thus triggering the cellular DNA repair system. Application of T4 endo V-containing preparations may ameliorate sun-induced damage in healthy persons as well as in patients defective in DNA repair capacity. We propose a rapid and efficient method for production of T4 endonuclease V without the need for phage inoculation of bacteria, thus allowing for easier and cost-effective manufacturing of T4endo V of the purity required by the GMP standards for medicinal preparations for use in humans.

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