ABSTRACT
T4 endonuclease V is specific to cyclobutane-pyrimidine dimers produced by UV-irradiation. When applied topically in mammals, it induces strand breaks in the altered DNA, thus triggering the cellular DNA repair system. Application of T4 endo V-containing preparations may ameliorate sun-induced damage in healthy persons as well as in patients defective in DNA repair capacity. We propose a rapid and efficient method for production of T4 endonuclease V without the need for phage inoculation of bacteria, thus allowing for easier and cost-effective manufacturing of T4endo V of the purity required by the GMP standards for medicinal preparations for use in humans.