In Vitro Plant Regeneration from Turkish Grasspea (Lathyrus Sativus L.) Using Immature Zygotic Embryo Explant

ABSTRACT

Grasspea (Lathyrus sativus L.) is an important grain legume crop cultivated in many countries of the world as animal feed. There is need to improve the plant through various available techniques. The study is aimed to develop an efficient and reproducible protocol for in vitro rapid and large-scale propagation of the plant from immature zygotic embryos using various concentrations of Thidiazuron (TDZ) and 6 benzylaminopurine (BAP)- α Naphthalene acetic acid (NAA) with and without ascorbic acid. The results showed that both TDZ and BAP-NAA without ascorbic acid were ineffective to induce shoot regeneration from explants due to excretion of phenolic compounds. The TDZ (with ascorbic acid) was more potent for axillary shoot regeneration compared to BAP-NAA (with ascorbic acid) with the highest shoot regeneration on MS medium containing 0.45 mg/l TDZ. The shoots regenerated on MS medium containing 0.45 mg/l TDZ (with ascorbic acid) were rooted on MS medium supplemented with 0.30, 0.60, 0.90 and 1.20 mg/l NAA. The best rooting was achieved on MS medium containing 0.90 mg/l NAA. It was not difficult to acclimatize all of the rooted plants in soil in greenhouse.

Keywords:

• immature embryos
• grass pea
• Lathyrus sativus L.
• axillary shoot regeneration
• rooting