Researches Concerning In Vitro Conservation of the Rare Plant Species Dianthus Nardiformis Janka

ABSTRACT

In our work, we studied the possibility to use in vitro methods for the preservation of the rare species from the Red List of Romanian Flora-Dianthus nardiformis Janka.

The plant material (whole plants and seeds) was collected from 3 populations from Natura 2000 sites.

The in vitro cultures starting from single node stem fragments had to face with high contamination degree with fungi. Despite this fact, using different methods of disinfection, culture media and combination of growth factors, aseptic tissues cultures and micropropagation were successfully performed. The seeds were less infected and are most suitable for in vitro tissues cultures initiation.

The rate of regeneration was evaluated after 2 months and the tissues cultures were used for the plant production and in the future for medium-term and long- term preservation approaches.

Keywords:

• Dianthus nardiformis
• in vitro preservation
• Natura 2000