Decolorization of Synthetic Dye Reactive Blue 4 by Mycelial Culture of White-Rot Fungi Trametes Versicolor 1

ABSTRACT

The industry is a major source of pollution for water ecosystems. Industrial production of textile, cellulose and various chemicals is connected with synthetic dyes usage. The discharged effluents could have a hazardous influence on the environment. The biological treatment for synthetic dyes removal is a very perspective, environmentally protective and low cost approach for solution of such problems. One of the often used and very important in dyeing of cellulosic fabrics and textile industry dyes is an anthraquinone-base chlorotriazine dye, known as Reactive Blue 4.

Decolorization of Reactive Blue 4 by Trametes versicolor strain 1 was investigated. The experiments were carried out with different concentrations of dye (50mg/l and 125mg/l) and glucose (1, 2 and 3%) in a medium. The enzyme activity of laccase (EC 1.10.3.2) was measured during the process of decolorization. It was shown that there is a direct correlation between the observed enzyme activity and the investigated process effectiveness. It was established that the best conditions for laccase production and decolorization of 125mg/l Reactive Blue 4 dye are in medium containing 3% glucose. In these conditions 90% Reactive Blue 4 was decolorized in 384 hours.

Keywords:

• decolorization
• laccase
• Reactive Blue 4
• Trametes versicolor