Study of Immobilized Microbial Composition on the Reclamation Process of Heavy Metal Polluted Soils

ABSTRACT

Heavy metal pollutions are in certain cases phytotoxic. Bioremediation can sometimes be realized with the using of microorganisms for “removing” of problematic pollutions from soil and underground water by helping the phytoremediation processes. The purpose ot the study is to determine the influence of biotransformed lignites' derivatives (HA) alone and as an agent for immobilization of micro fungi upon some bioremediation processes in heavy metal polluted soils. The pot vegetative test is realized with Ap horizons of polluted soils from the regions of Lead-Zink Procesing Plant Plovdiv (Alluvial-Meadow, Eutric Fluvisol, FAO) HA are a matrix for immobilization of T.viride and T. harzianum (10⁵CFU/ml solution), at a background treatment with Fe-zeolite. Test plants—garlic and grass mixture. A strong influence of the studied meliorating agents—bioproducts - upon bioremediation processes in polluted soil is observed. A good effect of immobilized micro flora on bioremediation processes is determined. The total content of the accumulated heavy metals from the test culture demonstrates the possibility of using HA as an immobilizing factor for T.viride and T. harzianum during rehabilitation of the productivity of heavy metal polluted soils.

Keywords:

• bioremediation
• heavy metal
• humic substances
• Trichoderma sp.