Molecular Marker Characterization of Breeding Lines Derived from Helianthus Annuus X Helianthus Bolanderi Inter-Specific Hybrids

ABSTRACT

Interspecific hybridization was used for developing new diversity in cultivated sunflower. As a result new lines with important economical traits were obtained. Two new breeding lines, 2530R and 2534R, that harbor several new traits, including resistance to downy mildew, were analyzed in this study. Both inbred lines originated from interspecies hybrid obtained by cross between Helinathus annuus L. and Helianthus bolanderi Gray, a wild annual diploid species from genus Helinathus. The genomic constitution of the two inbred lines was determined trough microsatelitte analysis. The polymorphism of 59 microsatellite markers, uniformly covering the sunflower genome, was assessed in lines 2530R and 2534R, line HA89A and Helianthus bolanderi Gray accession. Twenty one SSR loci that originate from the Helianthus bolanderi were obtained in the two inbred lines, thirteen in line 2530R and eleven in line 2534R.

This study is launched to the identification of chromosome regions, which being introgressed in lines 2530R and 2534R from the wild species, and underlie new agronomic traits.

Keyword:

• sunflower
• interspecific hybrid
• H.bolanderi
• microsatellite analysis
• SSR markers