Thermodynamic Analysis and Molecular Modeling of Rapana Venosa Hemocyanin—Functional Unit RVH2-E

ABSTRACT

pH-T diagram is typical “phase portrait” for stability of functional unit RvH2-e. Using different techniques the T-transition curves at different pH for RvH2-e were analyzed and the parameters of the thermodynamic functions were obtained. Increasing temperature and within the T range 25–55°C the reversibility increases and “opens a reversibility window” within the range of pH 5.5–9.0, for which were calculate at standard temperature the thermodynamic functions ΔH^o and ΔG^o_exp.

Molecular modeling of correct 3D structure of functional unit RvH2-e was done which allows us to fix most probably position of missing 9 residues now presented in existed x-ray model at very poor resolution of 3.30Å.

Keyword:

• Rapana venosa Hemocyanin functional unit RvH2-e
• Circular dichroism spectra
• Reversible denaturation
• Thermodinamic characteristics
• 3D- structure