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ABSTRACT
Glycotoxins, products of Maillard's reaction, are complex chemical substances. They are Introduced by food or developed in the human organism. They have negative effect on different structural and functional activities of important biological molecules in both cells and extracellular matrix. A model glycotoxin 3-hydroxy1-methylpyridinium hydrochloride (HMP.HCl) was synthesized. HMP.HCl was derived from 3-hydroxypyridine by methylation with methyliodid. Both the model glycotoxin and the starting 3-hydroxypyridine were investigated on cell cultures McCoy-Plovdiv and HepG-2 for cytotoxicity and genotoxicity activities. The test agents were used in a range of concentrations from 20 to 0.2 mg/ml. Quantity cytotoxicity assessment was determinated by NR- and MTT assays and genotoxicity by Micronucleus assay. The higher cytotoxicity was determined for HMP. HCl compared to 3-hydroxypyridine, but no genotoxicity was determined without an exogenous activation. The water solution from the modifying compound HMP. HCl strongly decreased medium pH. Correlation was determined between medium pH and concentrations of test compound. We suggest that high acidity of culture medium causes the higher cell death.