Allele Variation of Seven Housekeeping Genes in Twenty-One Dairy Enterococci

ABSTRACT

A collection of twenty-one dairy Enterococci isolated from cheese starters was subjected to phylogenetic analysis with a recently proposed new molecular method called multilocus single-strand conformation polymorphism (MSSCP). The obtained set of allele profiles related to the determined loci was presented through their frequencies. A classification analysis was applied in order to obtain the variation between the alleles studied.

Concerning the clustering of some of the species after the applied classification the allele variation assessment defines two major groups corresponding to Ent. faecium and Ent. faecalis. The applied method for phylogeic analysis through UPGMA has good discriminative power between the strains of the studied species.

Keywords:

• MSSCP
• allele variation
• classification analysis