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ABSTRACT
Preparations, containing humic substances become more and more popular in modern technologies for organic farming during the past years.
The purpose of the study is to determine both the influence of the supporting agent—modified zeolites—during biodestruction of lignite by T.harzianum, and the possibility for using the formed humic substances as a matrix for immobilization of enzyme systems.
The influence of chelate form of Fe, Cu, Mn, Zn, Co, Ca, Mg in composition with natural zeolite (clinoptilolite—Eastern Rhodopes, Bulgaria—ammonia form) upon the biotransformation of humic substances from lignite is studied. Lignite from Stanyantsy Mine is inoculated with T.harzianum 105 CFU/g; modified zeolites with concentration 20g/100g lignite are added to the medium.
Research data show that N-zeolites, modified with Cu, Zn, Mn and a combination of them, and used as a supporting agent during the biotransformation of lignite by T.harzianum, affect the humification and the formation of enzyme systems depending on the concentration of the saturating micro element. Zinc ions have a lower influence, and manganese ions slow down the humificaion to some extent. Zeolite components, modified with Mn increase the activity of polyphenol oxidase and decrease the activity of peroxidase.
Zeolite enrichment with Zn increases the activity of polyphenol oxidase and peroxidase, and the increased concentration of Cuions in zeolites lead to an increased activity of polyphenol oxidase and peroxidase.