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ABSTRACT
An improved HPLC method for quantitative determination of galanthamine, lycorine and norgalanthamine was developed. HPLC separation was achieved using a reversed phase C18 column Symmetry® and a gradient system with acetonitrile as an organic phase and 1% (w/v) ammonium acetate buffer adjusted to pH 6.6 with acetic acid (flow rate 0.3–0.5 ml/min.). The calibration curves were linear from 5 to 150 μg/ml (r2>0.99). The reliability of the proposed system was proved through reproducibility test with alkaloids extracts from in vitro shoot-clumps culture obtained from Leucojum aestivum L. and Pancratium maritimum L.