Molecular and Cytogenetic Criteria for Identification of Serum-Free Cell Cultures

ABSTRACT

The information about the authenticity of newly or already established animal cell cultures is essential. By using DNA analysis six cell lines has been investigated: 3T3, HeLa, McCoy, HEp-2, McCoy-Plovdiv and HEp-2-Plovdiv E. For the last four was made cytogenetic analysis confirmed that serum-free cell strains McCoy-Plovdiv and HEp-2-Plovdiv E are originally derived from McCoy and HEp-2 cell lines. PCR—analysis demonstrated that 3T3 cells are mouse originally, HeLa, HEp-2 and HEp-2- Plovdiv E are with the human origin and McCoy and McCoy-Plovdiv are hybrid cells carrying mouse and human genes in their genome.

Keywords:

• animal cell lines
• serum-free cell cultures
• McCoy-Plovdiv
• HEp-2-Plovdiv E
• cross-contamination