Some Aspects of Carbohydrate Metabolism and Production of Glycosyltransferases from Mutant Strain Leuconostoc Mesenteroides M2860

ABSTRACT

The production of glycosyltransferases (GTFs) from constitutive mutant strain Leuconostoc mesenteroides M2860 was studied. When grown in glucose medium in the absence of sucrose Leuc. mesenteroides M2860 produced low, but detectable GTF activity. Much of the GTF activity (81%) in sucrose grown cultures was located in the cell pellets. Extracellular and cell associated activities were determined when run on SDS-PAGE for in situ activity detection by periodic acid-Schiff s staining. In situ analysis showed single band corresponding to 180 kDa molecular size in supernatant and enzyme concentrate, received by cultivation on glucose media. The enzyme concentrate and supernatant fraction obtained by fermentation on sucrose media showed three bands corresponding to 180 kDa, 120 kDa and 86 kDa.

Keywords:

• constitutive mutant
• glycosyltransferases
• Leuconostoc mesenteroides