Evaluation of different pretreatments on microbial transformation of saponins in Dioscorea zingiberensis for diosgenin production

Abstract

In order to evaluate the effects of different pretreatments on microbial transformation of saponins in Dioscorea zingiberensis (DZW), various methods have been systematically studied on a large scale. Five pretreatments, including physical separation, catalytic solvent extraction, ultrasonic fermentation, complex enzymatic hydrolyzation and enzymatic saccharification, were performed on DZW. Compared with other methods, complex enzymatic hydrolyzation significantly improved the efficiency of microbial transformation. Due to the pretreatment, a diosgenin yield of 92.6%, and diosgenin accumulation of 27.3 mg/g DZW were achieved. The high efficiency of this method was attributed to the separation of 84.3% starch and 76.5% fibre from DZW in the form of a sugar. Analysis of saponins in this microbial transformation process showed that the residual rates of the intermediate products were much lower than those obtained from other pretreatments. The results demonstrate that complex enzymatic hydrolyzation is a practical and effective pretreatment method for production of diosgenin from DZW in a microbial transformation way.

Keywords:

• saponins
• diosgenin
• microbial transformation
• pretreatment

Additional information

Funding

This work is financially supported by the National Natural Science Foundation of China [grant number 21206093], [grant number 21206125]; the Zhejiang Provincial Natural Science Foundation of China [grant number LQ12E08005].