Genetic diversity assessment of Vitis ficifolia Bge. populations from Henan province of China by SRAP markers

Abstract

Eighteen sequence-related amplified polymorphism (SRAP) primer combinations were used to assess the genetic diversity of 126 individuals from five different geographical populations of Vitis ficifolia Bge. The numbers of bands scored per primer combination ranged from 8 to 27, with an average of 18.6 bands. At the population level, the percentage of polymorphic bands (PPB), Nei's gene diversity index (H) and Shannon's information index (I) were the highest in the Shihe (Xinyang) population (77.31%, 0.1987, 0.2805) and the lowest in the Linzhou (Anyang) population (55.82%, 0.1112, 0.1727). At the species level, PPB, H and I were 80.56%, 0.2129 and 0.3075, respectively. The genetic differentiation coefficient (G_ST) was 0.2055 and the gene flow (N_m) was 1.9328, indicating strong intra-population genetic differentiation. Based on the unweighted pair group method based arithmetic average clustering diagram, the five studied populations may be divided into three groups. The clustering results were almost in accordance with the populations’ geographical distribution.

Keywords:

• genetic diversity
• Vitis ficifolia Bge.
• populations
• SRAP

Acknowledgements

We are grateful to Prof. Dalong Guo at Henan University of Science and Technology for many helpful comments on previous drafts.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was financially supported by China Agriculture Research System [grant number CARS-30]; Crop Resources Protection Program of China Ministry of Agriculture [grant number 2130135-34]; Key Laboratory of the China Ministry of Agriculture on Fruit Breeding Technology [grant number 10104000].