Chloroplast DNA haplotype variation within two natural populations of wild emmer wheat (Triticum turgidum ssp. dicoccoides) in southern Turkey

Abstract

Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is the progenitor of cultivated emmer wheat (ssp. dicoccum) and durum wheat (ssp. turgidum conv. durum). Because of its full interfertility with domesticated emmer wheat, this wild species can serve as one of the most important genetic resources to improve durum as well as bread wheat. To elucidate the magnitude of genetic variation within a population of wild emmer wheat, variation of chloroplast DNA was investigated using 91 plants, in total, collected from two natural habitats in southern Turkey. Allelic variation at 24 microsatellite loci in the chloroplast genome was investigated using these samples. Allelic variations were observed at 15 microsatellite loci. The number of alleles per locus was the same in the two populations, ranging from 1 to 4 with an average of 2.17. The estimated diversity indices (H) were also very close ranging from 0.00 to 0.70 with an average of 0.28 and 0.29 for the two populations. Based on the observed allelic variation at all chloroplast microsatellite loci, a total of 23 chloroplast haplotypes (plastotypes) were identified. Only two plastotypes were shared in common between the two natural populations, indicating that the two populations are highly differentiated. Furthermore, uneven micro-geographic distribution of plastotypes was found within each population, suggesting limited rate of migration (seeds dispersal rate) in this species. Our study demonstrated the presence of a high level of genetic diversity between and within highly structured populations of wild emmer wheat in southern Turkey.

Keywords:

• natural habitat
• within population variation
• chloroplast DNA haplotype
• microsatellite
• wild emmer wheat
• Triticum turgidum ssp. dicoccoides

Acknowledgements

We thank Dr Chiharu Nakamura, professor Emeritus at Kobe University, for his valuable suggestions in the preparation of this manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

This work was supported in part by Japan Society for the Promotion of Science (JSPS) [grant-in-Aid number 15255012], [grant-in-Aid number 25640102].

Supplemental data

Supplemental data for this article can be accessed online http://dx.doi.org/10.1080/13102818.2015.1012648.