Evaluation of genetic diversity on Prunus mira Koehne by using ISSR and RAPD markers

Abstract

Peach (Prunus mira L.; 2n = 16), which originated in China, is a fruit tree widely cultivated all over the world with high economic value. Prunus mira Koehne is a wild species distributed in Tibet with special medicinal and ecological values. To conserve its breeding and germ plasm resources, two DNA-based molecular markers, inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD), were used for genetic diversity evaluations of 48 samples from three regions, including Tibet (XZ), Henan Province (HN) and Heilongjiang Province (HLJ), in this study. High levels of polymorphism were observed: 77.80% (ISSR) and 72.73% (RAPD), which indicated that ISSR and RAPD were efficient methods to detect the genetic diversity of P. mira Koehne. The samples from Tibet preserved higher genetic diversity and most genetic variations occurred among populations. There was no significant relationship observed between the genetic distance of populations and their geographic distribution. For conservation of P. mira Koehne, ex situ conservation is a suitable strategy that needs to be established in the future.

Keywords:

• Prunus mira Koehne
• genetic diversity
• ISSR
• RAPD

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by the Fundamental Research Funds for the Central Universities [grant number 2572015DA03], [grant number DL13EA08-02]; the National Natural Science Foundation of China [grant number 31170568], [grant number 31201594].