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ABSTRACT
In this work, we present a novel electroporator which is capable of generating single and bursts of high power (3 kV, 60 A) square wave pulses of variable duration (100 ns to 1 ms) with predefined repetition frequency (1 Hz to 3.5 MHz). The proposed synchronized crowbar implementation ensures a constant pulse rise and fall times, which are independent from the load, thus highly relevant in electroporation. The electroporator was successfully tested for the inactivation of the human pathogen Candida albicans. The device is compatible with standard commercial electroporation cuvettes.
Acknowledgments
This work was performed using the joint facilities of the Vilnius Gediminas Technical University, High Magnetic Field Institute (Vilnius, Lithuania) and the Nature Research Centre, Laboratory of Biodeterioration Research (Vilnius, Lithuania).
Disclosure statement
The authors do not have any conflict of interest. All applicable international, national and/or institutional ethical guidelines were followed.
