Isolation of a novel leucine-rich repeat receptor-like kinase (OsLRR2) gene from rice and analysis of its relation to abiotic stress responses

ABSTRACT

Leucine-rich repeat receptor-like kinase protein (LRR-RLK) is involved in a wide range of biological pathways. So far, the function of LRR-RLK in the growth, developmental processes and various external stimuli has still not been clearly elucidated in rice (Oryza sativa L.). To understand the mechanism(s) underlying stress response and to discover novel stress-tolerance genes in rice, we analysed a global genome expression profiling of the indica cultivar Pei'ai 64S subjected to cold, drought or heat stresses. Expression profiles were obtained for leaf and panicle tissues at seedling, booting and heading stages from plants under no stress, or cold, drought or heat stress, using the GeneChip Rice Genome Array (Affymetrix) representing 51279 transcripts from japonica and indica rice. We identified a gene, OsLRR2 (Oryza sativa L. leucine-rich repeat receptor-like kinase 2, GenBank accession: EAZ02952.1), which was highly up-regulated under cold and drought stress. In order to study its function in stress tolerance, we cloned the cDNA of the gene through amplification by reverse-transcription polymerase chain reaction. Sequence analysis showed that the cDNA encodes a protein of 375 amino-acid residues with molecular weight of ≈40.62 kD and pI of ≈5.75. The sequence databases search found that the open reading frame of OsLRR2 contained a leucine-rich repeats domain. Analysis of the putative promoter region for candidate cis-regulatory elements identified five matches to cis-elements related to stress responses, suggesting that OsLRR2 could be considered a new candidate gene involved in stress tolerance in rice.

KEYWORDS:

• Oryza sativa L.
• abiotic stress
• microarray
• gene cloning
• cold and drought stress
• LRR-RLK

Acknowledgment

We thank Wei Xu and Xiaomei Jia for offering help for revising this manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

Department of Science and Technology of Sichuan Province-This study was supported by the Science and Technology Department of Sichuan Province (an international co-operation project) granted to RJ Chen [grant number 2015HH0032].