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Article; Medical Biotechnology

No association between Id2 gene methylation and tetralogy of Fallot: a case-control study in China children

, , , &
Pages 671-678 | Received 26 Jun 2017, Accepted 16 Mar 2018, Published online: 24 Mar 2018
 

ABSTRACT

The aim of this case-control study was to investigate the correlation between the methylation levels of inhibitor of DNA binding 2 (Id2) gene and the incidence of tetralogy of Fallot (TOF) in children. The study included 31 TOF and 32 healthy control children. The methylation status of the Id2 gene was determined with time-of-flight mass spectrometry. Peripheral blood indices were detected, and their correlations with Id2 gene methylation were analyzed. Methylation analysis showed that there was no significant difference in the Id2 gene methylation level between the control and TOF groups (P > 0.05). After controlling the factors of gender, age, height and body weight, the analysis showed that there was no correlation between the methylation levels at each Id2 site and the red blood cells (RBC), hemoglobin (HGB) or hematocrit (HCT) (P > 0.05). However, the methylation levels at the CpG-16 and CpG-18.19 sites of the Id2 gene were negatively correlated with the mean corpuscular hemoglobin (MCH) (r = −0.337, P = 0.009; r = −0.392, P = 0.002) and mean corpuscular hemoglobin concentration (MCHC) (r = −0.363, P = 0.005; r = −0.286, P = 0.028), respectively. Our results suggest that the Id2 methylation might not be associated with the incidence of TOF. These results might contribute to the understanding of the etiology and mechanism of TOF in clinic.

Acknowledgements

This research was supported by the Natural Science Foundation of Heilongjiang Province under grant number H201412 and the Postdoctoral Fund of Heilongjiang Province under grant number 2013LBH-Z13152.

Disclosures statement

All authors declare no financial competing interests.

All authors declare no non-financial competing interests.

Additional information

Funding

This research was supported by the Natural Science Foundation of Heilongjiang Province [grant number H201412]; Postdoctoral Fund of Heilongjiang Province [grant number 2013LBH-Z13152]