Mechanisms of early- and late-feathering in Qingyuan partridge chickens

Abstract

Long non-coding RNAs (lncRNAs) are important in feather development and feathering patterns, but few studies on this subject have been conducted in chickens. To understand the follicle development and feathering phenotypes, lncRNA expression profiles in chicken wing skin were determined and combined with previously determined miRNA and mRNA expression profiles of chicken wing skin. We then predicted some regulatory networks among differentially expressed mRNAs, miRNAs and lncRNAs using bioinformatics. Compared to chickens with no feathers growing out (N1 group), 778 lncRNAs were differentially expressed in early-feathering chicks with primary feathers more than 2 mm longer than primary-covert feathers (F1 group), and 443 lncRNAs were differentially expressed in late-feathering chicks with primary feathers shorter than primary-covert feathers (L2 group). Only 45 lncRNAs were differentially expressed between F1 and L2 (fold-change > 2, q < 0.01). The targets of differentially expressed lncRNAs were involved in multiple processes related to feather growth and development. Integrated analysis of lncRNAs, miRNAs and mRNAs showed that 16 pairs negatively and 17 pairs positively interacted in feather formation. XLOC_045182 might inhibit early- and late-feather formation and feather phenotype via FK1L. 107052435 might negatively regulate feather growth and development via gga-miR-31-5p. 107052611 might restrict feather development by regulating SHH expression and XLOC_235660 might have a positive effect on feather development via FGF10.

Keywords:

• LncRNAs
• skin tissues
• chicken

Acknowledgements

We thank the Hengchuang Gene Company for high throughput sequencing and technical support. We thank Jeremy Allen, PhD, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.

Disclosure statement

The authors declare that they have no competing interests.

Additional information

Funding

This work was supported by the Key Project of Guangdong Province (2016B020233007), the Engineering Centre of Animal Breeding in Guangdong province (2017.1649) and Guangdong Provincial key Laboratory of Animal Molecular Design and Precise Breeding (2019B030301010). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.