Transcriptome sequencing and expression profiling of genes involved in daylily (Hemerocallis citrina Borani) flower development

Abstract

Daylily (Hemerocallis citrina Borani), a widespread ornamental plant around the world, has been considered as an ideal model to study flower development recently. To initiate the research to comprehensively investigate the molecular mechanism of the flower development of daylily, we performed a transcriptome sequencing study to establish the transcriptional profiles of this plant at different flower developmental stages. Briefly, we divided the flower development of daylily into six stages, and performed transcriptome sequencing for the samples collected from each stage. A total of 56,598 unique transcripts were obtained, of which 35,424 ones (about 62.6%) encode proteins with known functions based on homology searches against well-studied model plants. The expression profiles of these genes showed that 7,395 genes were differentially expressed during flower development. Further functional enrichment analysis for these differentially expressed genes revealed that they are enriched in the processes of translation, oxidation-reduction, and cell wall metabolism, suggesting the critical roles of these processes in daylily flower development.

Keywords:

• Hemerocallis citrina
• RNA-Seq
• flower development
• ROS
• cell wall

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Natural and Science Foundation of China (grant number 31302019) and China Postdoctoral Science Foundation (grant number 2016T90307 and 2015M571430).