Genome-wide identification and expression pattern of alkaline/neutral invertase gene family in Dendrobium catenatum

Abstract

Alkaline/neutral invertase (NI) proteins irreversibly break down sucrose into glucose and fructose and play a vital role in carbohydrate and plant growth and development. Currently, a comprehensive understanding of NI genes in Dendrobium catenatum is lacking. After genome-wide identification and prediction, nine DcNI genes were identified and named DcNI1–DcNI9. Through comprehensive analysis of gene structure, amino acid sequence, conserved motif and phylogenetic tree, it was found that the DcNI gene family has a typical conserved region and its amino acid sequence is similar to other plant NI genes. However, DcNI3 lost amino acid sequences at both the N-terminus and C-terminus, while DcNI8 lost amino acids at the C-terminus. Phylogenetic analysis revealed that the DcNI family could be divided into α and β groups. It reveals conservation and divergence of DcNIs. Predictive analysis of cis-acting elements in the promoter region reveals the potential response of DcNI protein to hormones and environmental stress and suggests it to be widely involved in growth and development. DcNIs were differentially expressed in different tissues such as roots, stems and leaves. In particular, DcNI4 was highly expressed in the stem. They also had a differential expression response under drought, cold, P1 fungus infection and jasmonic acid (JA) treatment. To the best of our knowledge, this is the first genome-wide analysis of the DcNI gene family in D. catenatum. This study will provide important and primary information for the functional study of the DcNI gene family and the polysaccharide synthesis pathway in D. catenatum.

Keywords:

• Alkaline/neutral invertase
• Dendrobium catenatum
• gene family
• polysaccharide biosynthesis

Disclosure statement

The authors declare no conflict of interest.

Author contributions

Conceptualization, C.L. and L.Z.; methodology, C.L.; formal analysis, C.L.; investigation, C.L.; writing – original draft preparation, C.L.; writing – review and editing, C.L., L.Z. J.S., H.X., X.C., Y.Z. and J.Y.; supervision, L.Z.; funding acquisition, J.S and L.Z. All authors have read and agreed to the published version of the manuscript.

Data sharing

All data that support the findings reported in this study are available from the corresponding author upon reasonable request.

Additional information

Funding

This work was funded by National Key R&D Program of China (2017YFC1702201) focusing on wild imitation cultivation and stress response mechanisms of Dendrobium, National Natural Science Foundation of China (31870310) focusing on epigenetic mechanism of Dendrobium epiphytic lifestyle, the State Key Laboratory of Subtropical Silviculture (ZY20180206) focusing on metabolic mechanism of environmental fitness in Dendrobium. The funding agencies were not involved in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

Funding

This work was funded by National Key R&D Program of China (2017YFC1702201) focusing on wild imitation cultivation and stress response mechanisms of Dendrobium, National Natural Science Foundation of China (31870310) focusing on epigenetic mechanism of Dendrobium epiphytic lifestyle, the State Key Laboratory of Subtropical Silviculture (ZY20180206) focusing on metabolic mechanism of environmental fitness in Dendrobium. The funding agencies were not involved in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.