Transcriptome analysis of soybean leaves response to manganese toxicity

Abstract

As an essential micronutrient, manganese (Mn) participates in diverse processes during plant growth and development. Excess accumulation of Mn in plants is toxic, and soybean (Glycine max) growth and production are severely limited by Mn toxicity. However, the molecular basis in adaptation to Mn toxicity for soybean remains largely unknown. In this study, RNA-seq analysis on soybean leaves was conducted, more than 44 million reads were generated, and a total of 38,022 expressed genes were identified. Compared to control, 2258 differentially expressed genes (DEGs), including 744 up-regulated and 1514 down-regulated ones, were obtained. Cellular process, cell part and binding function were the most enriched terms by GO analysis. Furthermore, 49 DEGs were identified in plant hormone signal transduction pathways by KEGG analysis. Among them, Mn toxicity up-regulated AHK, PIF, JAZ and TGA family DEGs might play important roles in the adaptation of soybean leaves to Mn toxicity.

Supplemental data for this article is available online at https://doi.org/10.1080/13102818.2021.1950566 .

Keywords:

• RNA-seq
• manganese toxicity
• soybean
• leaves

Disclosure statement

The authors have declared that no competing interests exist.

Funding

This research was funded by the Natural Science Foundation of Guangdong Province (2018A030310057 and 2020A1515011570), Nanhai Youth Scholar Project of Guangdong Ocean University (002029001012), Program for Scientific Research Start-up Funds of Guangdong Ocean University (R17023 and R19031), Foundation of Education Department of Guangdong Province (2019KTSCX059), the Project of Science and Technology of Zhanjiang City (2020B01019), the National Natural Science Foundation of China (32002131).

Data availability statement

The data that support the findings of this study are openly available in Mendeley Data at http://doi.org/10.17632/p4sr6zh84h.1