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ABSTRACT
The family Sphaeriidae is a group of cosmopolitan freshwater clams that inhabit temporary and permanent fluvial and lacustrine environments around the world. In Chile, Sphaeriidae is represented by 11 species belonging to the genera Musculium, Pisidium and Sphaerium. In this study, the nuclear DNA content (or 2C-value) of Musculium argentinum (d’Orbigny, 1835) and Pisidium llanquihuense Ituarte, 1999 was estimated for the first time. Musculium argentinum showed a 2C-value of 3.7 ± 0.38 pg, whereas P. llanquihuense showed a 2C-value of 3.57 ± 0.36 pg. These 2C-values are within the range previously reported for Sphaeriidae (2.35–5.7 pg), which includes diploids and polyploids, amounting to 13 of the species examined around the world. Of the cytological data available for the family, the nuclear DNA content can have a unifying role in understanding the magnitude of the chromosome polymorphisms within this polyploid complex.
Acknowledgements
Technical equipment for microscopy and image analysis was supplied by Project FONDEF D98I-1044 and D02I-1095. A special acknowledgement to Esperanza Parada for her contribution to the knowledge of the Chilean freshwater bivalves. We also thank the reviewers for their useful suggestions.
Disclosure statement
No potential conflict of interest was reported by the authors.