68
Views
1
CrossRef citations to date
0
Altmetric
Short Communication

Anterograde transsynaptic tracing in the murine somatosensory system using Pseudorabies virus (PrV): A “live-cell”-tracing tool for analysis of identified neurons in vitro

, , , , , & show all
Pages 579-585 | Received 06 Jun 2007, Accepted 05 Jul 2007, Published online: 10 Jul 2009
 

Abstract

The Pseudorabies virus (PrV) strain Bartha is widely used as a tool for retrograde transneuronal tracing in mammals. Traced neurons can be identified in cell culture allowing the analysis of their physiological features (“live-cell”-tracing). Compared to PrV-Bartha, PrV-Kaplan is known for higher virulence and transsynaptic spread in both retrograde and anterograde direction. Herein we assess the authors assess PrV-Kaplan for transsynaptic anterograde “live-cell”-tracing. Following intranasal application in mice, labelled trigeminal and brainstem neurons could be identified in vitro. Detailed electrophysiological analysis indicated that viral infection did not affect neuronal properties, making PrV-Kaplan eligible for functional analysis of identified neurons within somatosensory systems.

The present address of Nils Damann is Departement Moleculaire Celbiologie, Katholieke Universiteit Leuven, Belgium.

This work was supported by the Deutsche Forschungsgemeinschaft (Graduiertenkolleg 736 “Development and Plasticity of the Nervous System: Molecular, Synaptic and Cellular Mechanisms,” University of Bochum, Bochum, Germany). This work was additionally supported by the Ruhr-University Research School funded by the DFG in the framework of the Excellence Initiative. The authors also thank Harry Bartel for his technical support with equipping patch-clamp setups.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.