Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii

Wieland Meyer Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, University of Sydney Western Clinical School at Westmead Hospital, Westmead, Australia

David M. Aanensen Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, London, UK

Teun Boekhout CBS Fungal Biodiversity Centre, Utrecht, The Netherlands

Massimo Cogliati Laboratory of Medical Mycology, Dip. Sanitá Pubblica-Microbiologia-Virologia, Università degli Studi di Milano, Milano, Italy

Mara R. Diaz Rosentiel School of Marine and Atmospheric Science, University of Miami, Miami, Fl, USA

Maria Carmela Esposto Laboratory of Medical Mycology, Dip. Sanitá Pubblica-Microbiologia-Virologia, Università degli Studi di Milano, Milano, Italy

Matthew Fisher Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, London, UK

Felix Gilgado Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, University of Sydney Western Clinical School at Westmead Hospital, Westmead, Australia

Ferry Hagen CBS Fungal Biodiversity Centre, Utrecht, The Netherlands

Sirada Kaocharoen Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, University of Sydney Western Clinical School at Westmead Hospital, Westmead, Australia; Inter-Department of Medical Microbiology, Graduate School, Chulalongkorn University, Bangkok, Thailand

Anastasia P. Litvintseva Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA

Thomas G. Mitchell Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA

Sitali P. Simwami Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, London, UK

Luciana Trilles Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, University of Sydney Western Clinical School at Westmead Hospital, Westmead, Australia; Laboratório de Micologia, Instituto de Pesquisa Clínica Evandro Chargas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil

Maria Anna Viviani Laboratory of Medical Mycology, Dip. Sanitá Pubblica-Microbiologia-Virologia, Università degli Studi di Milano, Milano, Italy

June Kwon-Chung Molecular Microbiology Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH, Bethesda, MD, USACorrespondence[email protected]

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Abstract

This communication describes the consensus multi-locus typing scheme established by the Cryptococcal Working Group I (Genotyping of Cryptococcus neoformans and C. gattii) of the International Society for Human and Animal Mycology (ISHAM) using seven unlinked genetic loci for global strain genotyping. These genetic loci include the housekeeping genes CAP59,GPD1, LAC1, PLB1, SOD1, URA5 and the IGS1 region. Allele and sequence type information are accessible at http://www.mlst.net/.

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Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii

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Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii

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