Biomarkers of oxidative stress and protein–protein interaction in chronic obstructive pulmonary disease

Abstract

Content: The increased oxidative stress in chronic obstructive pulmonary disease (COPD) patients is the result of increased inhaled oxidants, generated by various cells of the airways.

Objective: The investigation included measurements of malondiadehyde (MDA), uric acid, ascorbic acid, and matrix metalloproteinase-12 (MMP-12) in COPD patient. We also performed genetic analysis for protein–protein interaction (PPI) network.

Materials and methods: The study was conducted on healthy subjects with normal lung function (NS, 14 subjects) and 28 patients (Global Initiative for Chronic Obstructive Lung Disease (Gold) 1 and Gold 2) with COPD.

Results: There was significant (p < .001) increase in MMP-12, MDA and uric acid levels as compared to healthy controls. A significant (p < .001) decline in ascorbic acid level was observed in COPD patients. The PPI was found to be 0.833 which indicated that proteins present in COPD are linked.

Discussion and conclusion: This study suggests oxidative stress plays an important role in COPD and the PPI provide indication that proteins present in COPD are linked.

Keywords:

• Chronic obstructive pulmonary disease
• MMP-12; MDA
• uric acid
• ascorbic acid
• oxidative stress
• PPI network

Acknowledgements

PKM thanks Amity University Uttar Pradesh, Noida for providing necessary facility for completion of this research work.

Disclosure statement

No potential conflict of interest was reported by the authors.