https://orcid.org/0000-0001-7767-8491
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Abstract
Objective
To investigate the effect of electrical pulse stimulation (EPS) on lipid accumulation and alteration of fatty acid-related enzymes in C2C12 myotubes incubated with fatty acids.
Methods
Mouse C2C12 myotubes were incubated with oleic acid and palmitic acid, and differentiated C2C12 myotubes were treated with EPS, oil-red O (ORO), BODIPY staining and triglyceride (TG) content were examined. Total RNA was isolated, and real-time polymerase chain reaction analysis was performed.
Results
(1) EPS decreased TG content (p < .01). (2) EPS significantly induced the mRNA expression of FAD/CD36 (p < .05), FATP4 (p < .001), FABP1 (p < .01) and FABP5 (p < .01). (3) EPS significantly inhibited the mRNA expression of fatty acid synthase (p < .01). (4) Adipose triglyceride lipase and hormone-sensitive lipase expression were significantly elevated (p < .001), and induced the mRNA expression of CPT1 (p < .01), ACOX1 (p < .05), UCP3 (p < .05) and PPARα (p < .001) after EPS.
Conclusion
EPS reduced lipid droplet accumulation; enhanced CD36, FATP4, FABP1 and FABP5 expression; inhibited C2C12 myotube fatty acid re-esterification; and promoted fatty acid oxidation in C2C12 myotubes.
Disclosure statement
No potential conflict of interest was reported by the authors.