Some sulfonamides as aldose reductase inhibitors: therapeutic approach in diabetes

Abstract

In this study, aldose reductase (AR) was purified from sheep kidney using chromatographic methods and examined the interactions between some sulfonamides and the enzyme. According to results, sulfonamides display effective inhibitor features for sheep kidney AR with IC₅₀ values in the range of 37.27–87.65 μM and K_is in the range of 25.72 ± 6.45 to 73.56 ± 17.49 μM. The sulfonamides displayed different inhibition mechanisms. It was found that studied all compounds displayed non-competitive inhibition type except for 5-chlorothiophene-2-sulfonamide (1). It showed competitive inhibition. Among these compounds, 2,5-dichlorothiophene-3-sulfonamide compound (2) was showed the most potent AR inhibitor (K_i: 25.72 ± 6.45). These compounds may be useful in the treatment of diabetic complications.

Keywords:

• Enzyme purification
• aldose reductase
• oxidative stress
• enzyme inhibition
• sulfonamides

Disclosure statement

No potential conflict of interest was reported by the author(s).