Determination of carnitine ester profile in the children with type 1 diabetes: a valuable step towards a better management

Abstract

Objective: This study is designed to investigate the levels of carnitine and acylcarnitines (ACs) in the children with diabetes type 1 compared to the healthy subjects.

Methods: Forty-two type 1 diabetic children and healthy subjects were recruited in the study, respectively. In addition to FBS and Hb A1C, free carnitine and ACs in butyl-ester form in the fasting blood samples were assessed by isotope dilution mass spectrometry for all diabetics and controls using the tandem mass spectrometry system.

Results: Diabetic patients had a higher level of C, C4, C6, C14, C18:2, and C18:2OH. Females had elevated C14:2 compared to the males. The C18:2 and C18:2OH levels were elevated as the Hb A1C level increased. The C18:2, C14OH were mostly increased in the prediabetic and diabetic patients, respectively.

Conclusion: Increased ACs level indicates the increased acyl-CoA intermediates for the fatty acids and amino acids oxidation.

Keywords:

• Acyl carnitine
• free carnitine
• metabolism
• type 1 diabetes
• children

Ethical approval

All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Disclosure statement

The authors deny any conflict of interest in any terms or by any means during the study. All the fees provided by research centre fund and deployed accordingly.

Availability of data and material

Data sharing not applicable to this article as no datasets were generated or analysed during the current study.

Author contributions

Dr. Parisa Rahmani, Dr. Jabar Lotfi and Dr.Ghobad Heidari: conceptualised and designed the study, drafted the initial manuscript, and reviewed and revised the manuscript.

Dr. Ruhollah Abolhasani and Dr. Fatemeh Sayarifard: Designed the data collection instruments, collected data, carried out the initial analyses, and reviewed and revised the manuscript.

Dr. Ali Mohebi, Dr. Ali Rabbani and Dr.Nahid Vafaei: Coordinated and supervised data collection, and critically reviewed the manuscript for important intellectual content.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.