Exosomal expression of RAB27A and its related lncRNA Lnc-RNA-RP11-510M2 in lung cancer

Abstract

Objectives

Examine the diagnostic role of serum exosomal RAB27A mRNA in lung cancer and evaluate the relation of LncRNAs to lung cancer in association to RAB27A mRNA in Egyptian population.

Methods

Exosomal RNA-based biomarkers RAB27A mRNA and Lnc-RNA-RP11-510M2.10 were selected based on bioinformatic methods, followed by RT-qPCR validation of their expression in serum of 20 patients with lung cancer, 10 patients with COPD and 10 healthy volunteers. we examined their expression in 10 bronchoalveolar lavage samples and assessed correlation with the serum levels.

Results

There was an inverse relationship between expression of serum exosomal RAB27A mRNA and Lnc-RNA-RP11-510M2.10 (r = −0.62, p = .00). Both serum exosomal RAB27A mRNA and Lnc-RNA-RP11-510M2.10 showed a significant positive and negative association with lung cancer patients respectively in comparison to patients with COPD and healthy persons (p < .001).

Conclusion

RAB27A mRNA and Lnc-RNA-RP11-510M2.10 could be used as diagnostic and prognostic biomarker tools for lung cancer.

Keywords:

• RAB27A
• Lnc-RNA-RP11-510M2.10
• exosomal RNA extraction
• lung cancer

Ethical approval and informed consent

Written informed consent was obtained from all the participants of this study, which was performed in accordance with Declaration of Helsinki, and was approved by the Ethics Committee of Ain Shams Faculty of Medicine, Egypt. This work was carried out in compliance with Ethical Standards.

Disclosure statement

All the authors; Ghada Mohamed Gamal El-Din, Fawzia Khalil Ibrahim, Hanan Hussein Shehata, Nehad Mohammed Osman, Omar Mohammed Abdel-Rahman and Marwa Ali, declared that they have no competing interest.

Additional information

Funding

This work was supported by the Ain Shams Faculty of Medicine Grant s Office [Grant No. 2017/7].