![Sample our Medicine, Dentistry, Nursing & Allied Health journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/5944/TOC-Subject-Sample-2021-Medicine-Dentistry-Nursing-Allied-Health.jpg"})
Abstract
Background
Diet macronutrient heterogeneity hinders animal studies’ data extrapolation from metabolic disorders to human diseases.
Objective
The present study aimed to evaluate different fat-diet compositions’ effect on inducing lipid/glucose metabolism alterations in mice.
Methods
Swiss male mice were fed for 12 weeks with five different diets: Standard Diet (ST), American Institute of Nutrition 93 for growth (AIN93G) high-butter/high-sugar (HBHS), high-lard/high-sugar (HLHS), and high-oil/high-sugar diet (soybean oil) (HOHS). Several parameters, such as serum biochemistry, histology, and liver mRNA expression, were accessed.
Results
The main findings revealed that the HLHS diet dramatically altered liver metabolism inducing hepatic steatosis and increased total cholesterol, triglycerides, VLDL, increasing liver CCAAT/enhancer binding protein (CEBP-α), Acetyl-CoA carboxylase (ACC) and Catalase (CAT) mRNA expression. Moreover, the HLHS diet increased glucose intolerance and reduced insulin sensitivity.
Conclusions
High-fat/high-sugar diets are efficient to induce obesity and metabolic syndrome-associated alterations, and diets enriched with lard and sugar showed more effective results.
Disclosure statement
The authors declare no conflict of interest.
Data availability statement
The authors confirm that the data supporting the findings of this study are available within the article [and/or] its supplementary materials.
Author contributions
VHDG study design, data analysis and drafting the article, DFL drafting the article, LMAB, LPO and FADG data analysis and acquisition of data, AMBP interpretation of data, SHSS, ALSG and LCF revising it critically for important intellectual content. Authors give final approval of the version to be submitted.
