Separation and Quantification of Tiliroside from Plant Extracts by SPE/RP-HPLC

Abstract

Kaempferol-3-O-β-D-(6″-E-p-coumaroyl)-glucopyranoside (tiliroside) has been isolated from many natural plants sources. This compound showed very interesting pharmacological activities, e.g., cytotoxic for human leukaemic cell lines and anti-complement activity. However, there is a lack of data concerning the presence of this compound in medical plants. In this work, a reproducible and quick method was elaborated for the separation and determination of tiliroside in methanolic extracts of the plants. Solid-phase extraction was applied as sample preparation technique for the purification of crude extracts from various species as well as for the isolation of tiliroside for further reversed-phase high performance liquid chromatography analysis. The content of tiliroside for various plant materials was compared.

Keywords::

• Tiliroside
• quantitative analysis
• solid-phase extraction
• RP-HPLC analysis