Bioautography with the Fungus Valsa ceratosperma in the Search for Antimycotic Agents

N. Islam Crop Protection Unit, Department of Zoology, Rajshahi University, Rajshahi, 6205, Bangladesh

S.A. Parveen Crop Protection Unit, Department of Zoology, Rajshahi University, Rajshahi, 6205, Bangladesh

N. Nakazawa Crop Protection Unit, Department of Zoology, Rajshahi University, Rajshahi, 6205, Bangladesh

A. Marston Institute of Pharmacognosy and Phytochemistry, University of Lausanne, Rajshahi, 6205, Switzerland

K. Hostettmann Institute of Pharmacognosy and Phytochemistry, University of Lausanne, Rajshahi, 6205, Switzerland

Abstract

A simple bioautographic agar spray assay has been developed using the plant pathogenic fungus Valsa ceratosperma as an indicator organism for the detection and activity guided fractionation of antifungal compounds by thin-layer chromatography. Besides silica gel G60 F₂₅₄ aluminium- and glass-backed plates, chemically modified silica gel (RP-18 F_254S, RP-18 WF_254S, RP-8, etc.) plates and various organic solvents were used to evaluate the compatibility of the agar spray technique. Two fungicides, Iprodione (Rovral®) and Mepronil (Bastak®), were tested to determine the sensitivity of the test fungus.

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Bioautography with the Fungus Valsa ceratosperma in the Search for Antimycotic Agents

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Bioautography with the Fungus Valsa ceratosperma in the Search for Antimycotic Agents

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