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ABSTRACT
The antibacterial activity of Glaucium grandiflorum. Boiss. & Huet, G. oxylobum. Boiss. & Buhse, and G. paucilobum. Freyn, native plants of Iran, was studied using the disk diffusion method and determination of minimum inhibitory concentration (MIC) values against Staphylococcus aureus. PTCC 1112, Streptococcus sanguis. PTCC 1449, Escherichia coli. PTCC 1330, Pseudomonas aeruginosa. PTCC 1074, and Klebsiella pneumoniae. PTCC 1053. The methanol and chloroform extracts of the aerial parts of G. grandiflorum., G. oxylobum., and G. paucilobum. exhibited concentration-dependent antibacterial activity against all tested bacteria. The methanol and chloroform extracts were more active against Gram-negative microorganisms (Escherichia coli., Pseudomonas aeruginosa., Klebsiella pneumoniae.). The chloroform extracts were found to be most effective against all tested strains.